This new hydrazone resin provides a convenient method for the synthesis of peptide hydrazides, which can be applied in ligation or fragment condensation technique. It was shown that the hydrazone linker can tolerate treatment with 5 % TFA/DCM thus permitting selective removal of Mtt or related acid-labile protecting groups. The use of preloaded resins is more convenient, permits to save time and in certain cases circumvents the racemization issues.
- Facile and efficient chemical synthesis of APET×2, an ASIC-targeting toxin, via hydrazide-based native chemical ligation; S.-J. Li, D.-L. Qu, Y.-H. Wang, Y. He, M. Wen, Q.-X. Guo, J. Shi and Y.-M. Li; Tetrahedron 2015; 71: 3363-3366. doi:10.1016/j.tet.2015.03.098
- Chemical synthesis of proteins using peptide hydrazides as thioester surrogates; J.-S. Zheng, S. Tang, Y.-K. Qi, Z.-P. Wang and L. Liu; Nature Protocols 2013; 8: 2483 doi:10.1038/nprot.2013.152